| First Author | Maass A | Year | 1994 |
| Journal | Biochem Biophys Res Commun | Volume | 202 |
| Issue | 3 | Pages | 1337-46 |
| PubMed ID | 8060311 | Mgi Jnum | J:19616 |
| Mgi Id | MGI:67765 | Doi | 10.1006/bbrc.1994.2077 |
| Citation | Maass A, et al. (1994) Mitogenic signals control translation of the early growth response gene-1 in myogenic cells. Biochem Biophys Res Commun 202(3):1337-46 |
| abstractText | Muscle is a major site of expression of the early growth response gene-1 (Egr-1). To investigate its role in muscle proliferation and/or differentiation we studied the effect of a variety of growth factors on cultured mouse muscle Sol8 cells. Three groups of responses could be distinguished: 1. AII, endothelin, phenylephrine, and PMA induced Egr-1 mRNA accumulation, but the message remained untranslated. These factors induced neither differentiation nor proliferation. 2. Insulin induced differentiation. It stimulated Egr-1 mRNA accumulation, but no translation into the Egr-1 protein was seen. 3. bFGF, PDGF BB, and FCS strongly induced DNA- and protein synthesis (i.e. proliferation) and Egr-1 mRNA accumulation. Only under these conditions was the message translated into protein. We conclude: 1. AII, endothelin, phenylephrine, and PMA elicit a nuclear response in Sol8 muscle cells which may lead to reprogramming of genes unrelated to differentiation or proliferation. 2. Differentiation induces a translational block of the Egr-1 mRNA which is only relieved by mitotic stimuli. 3. These results strongly suggest a pivotal role of Egr-1 in muscle proliferation and define translational control as a new mechanism of Egr-1 regulation. |
