| First Author | Siu G | Year | 1994 |
| Journal | EMBO J | Volume | 13 |
| Issue | 15 | Pages | 3570-9 |
| PubMed ID | 8062832 | Mgi Jnum | J:19770 |
| Mgi Id | MGI:67903 | Doi | 10.1002/j.1460-2075.1994.tb06664.x |
| Citation | Siu G, et al. (1994) A transcriptional silencer controls the developmental expression of the CD4 gene. EMBO J 13(15):3570-9 |
| abstractText | The appropriate expression of the CD4 glycoprotein is required for T-cell function and development. Here we define the transcriptional control elements in the CD4 locus that convey CD(4+)-specific expression of a marker gene in transgenic mice. Using nuclear run-on experiments, we have determined that the major mechanism for CD4 expression control during development is transcriptional. We have identified a developmental stage- and tissue-specific negative regulatory element in the first intron of the murine CD4 gene that has the characteristics of a transcriptional silencer. The CD4 silencer functions to inhibit marker gene expression at two different stages of T-cell development, as well as in non-T hematopoietic cells, and thus is the critical controlling element responsible for T-cell-specific, as well as developmental- and subclass-specific, expression. |
