| First Author | Plath K | Year | 1994 |
| Journal | Biochem Biophys Res Commun | Volume | 203 |
| Issue | 2 | Pages | 1188-94 |
| PubMed ID | 8093038 | Mgi Jnum | J:20329 |
| Mgi Id | MGI:68427 | Doi | 10.1006/bbrc.1994.2308 |
| Citation | Plath K, et al. (1994) Characterization of the proline-rich region of mouse MAPKAP kinase 2: influence on catalytic properties and binding to the c-abl SH3 domain in vitro. Biochem Biophys Res Commun 203(2):1188-94 |
| abstractText | The primary structure of mouse MAP kinase-activated protein (MAPKAP) kinase 2 contains a proline-rich N-terminal region which might function as a src-homology 3 (SH3) domain-binding motif in vivo. To demonstrate the ability of this region to bind SH3 domains, we analyzed the interaction of the SH3 domain of the protein tyrosine kinase c-abl with MAPKAP kinase 2. It is demonstrated, that the proline-rich region specifically binds c-abl-SH3 domain in vitro. Furthermore, it is shown, that deletion of this proline-rich region does not significantly influence the substrate binding properties of the enzyme when analyzed with the substrate small heat shock protein Hsp25. The data suggest that the proline-rich region of MAPKAP kinase 2 could interact with proteins containing SH3-domains also in vivo regulating its cellular localization and/or modulating its enzymatic properties. |
