| First Author | Duc-Goiran P | Year | 1994 |
| Journal | Exp Cell Res | Volume | 214 |
| Issue | 2 | Pages | 570-83 |
| PubMed ID | 7925651 | Mgi Jnum | J:20666 |
| Mgi Id | MGI:68742 | Doi | 10.1006/excr.1994.1295 |
| Citation | Duc-Goiran P, et al. (1994) Developmental control of IFN-alpha expression in murine embryos. Exp Cell Res 214(2):570-83 |
| abstractText | The expression of IFN-alpha transcripts was investigated in murine embryos, fetuses, and fetal annexes in mid and late pregnancy. We have shown by Northern blot analysis, reverse transcription-polymerase chain reaction, and in situ hybridization the presence of IFN-alpha transcripts in mouse placenta, fetus, and newborn. From the 14th day of gestation until birth, a typical IFN-alpha transcript (1.2 kb) is found in the fetus. A transcript of larger size (2.2 kb) appears near birth and is present in the newborn mouse. Fetal annexes between the 10th and 21st days of gestation also express IFN-alpha. From the 10th day until birth, the 1.2-kb IFN-alpha mRNA species is present, as well as unusually large transcripts: 4 and 7 kb. To localize IFN-alpha transcripts, in situ hybridization was performed, using 35S-IFN-alpha antisense RNA probe in comparison with the sense RNA probe. The tissue pattern of IFN-alpha transcription in fetuses shows a clear labeling of many epithelia, such as skin, ependyme, and intestine glandular epithelium. A possible relation with cellular differentiation is discussed. |
