| First Author | Norga K | Year | 1994 |
| Journal | Biochem Biophys Res Commun | Volume | 205 |
| Issue | 1 | Pages | 886-92 |
| PubMed ID | 7999127 | Mgi Jnum | J:21809 |
| Mgi Id | MGI:69715 | Doi | 10.1006/bbrc.1994.2747 |
| Citation | Norga K, et al. (1994) Multiple transcription start sites and 5' alternate splicing of murine IL-3 receptor beta-chain transcripts. Biochem Biophys Res Commun 205(1):886-92 |
| abstractText | The murine interleukin-3 receptor beta-chain genes, IL-3R beta IL-3 and IL-3R beta c, encode the signal transducing chains of the high affinity receptors for IL-3 and IL-3, GM-CSF and IL-5 respectively. Little is known about the regulation of their expression. To enable the study of the promotors of IL-3R beta IL-3 and IL-3R beta c, we have characterized their respective 5' untranslated regions using a modified 5' RACE protocol. Four classes of alternatively spliced transcripts were isolated that initiate in a 400 nt region upstream from a previously reported start site(1). The initially reported partial IL-3R beta IL-3 clone belongs to the first class of transcripts(2). The second class starts in the middle of an intron as defined by the first class. The 3rd and 4th class establish 2 novel splice donor sites. These results were confirmed by RNAse-protection assay. The complex organization as evident from our data establishes an experimental framework for future experiments aimed at the study of the promoters for the murine IL-3R beta genes. |
