| First Author | Tajima Y | Year | 1994 |
| Journal | J Reprod Fertil | Volume | 102 |
| Issue | 1 | Pages | 117-22 |
| PubMed ID | 7528277 | Mgi Jnum | J:21605 |
| Mgi Id | MGI:69541 | Doi | 10.1530/jrf.0.1020117 |
| Citation | Tajima Y, et al. (1994) Switching of mouse spermatogonial proliferation from the c-kit receptor-independent type to the receptor-dependent type during differentiation. J Reprod Fertil 102(1):117-22 |
| abstractText | Testicular cells composed mostly of germ cells and immature Sertoli cells from neonatal mice 2 and 5 days old were cultured to investigate germ-cell proliferation mediated by the c-kit receptor. The addition of antibody to block the interaction of the c-kit receptor with its ligand inhibited the proliferation of cultured spermatogonia from 5-day-old mice in a dose-dependent manner, but not from that of 2-day-old mice. The addition of anti-c-kit ACK2 monoclonal antibody also inhibited the proliferation of spermatogonia from 5-day-old mutant Sld/Sld mice but not of 5-day-old mutant Wv/Wv mice. The results indicate that c-kit-positive type A spermatogonia in the testes of 5-day-old mice require steel factor (kit ligand) for their proliferation, whereas self-renewal and differentiation of c-kit-negative primitive type A spermatogonia in the testes of 2-day-old mice do not. |
