| First Author | Greene DR | Year | 1995 |
| Journal | Int J Cancer | Volume | 60 |
| Issue | 3 | Pages | 395-9 |
| PubMed ID | 7829250 | Mgi Jnum | J:24059 |
| Mgi Id | MGI:71807 | Doi | 10.1002/ijc.2910600321 |
| Citation | Greene DR, et al. (1995) DNA ploidy and clonal selection in ras + myc-induced mouse prostate cancer. Int J Cancer 60(3):395-9 |
| abstractText | An important goal in prostate cancer research is to define specific molecular and cellular alterations that are associated with malignant progression. The mouse prostate reconstitution model is a relevant and useful system as it allows the study of early events in cancer progression under conditions where oncogene-initiated cells are surrounded by normal tissue. Using this model, activated ras and myc oncogenes are introduced into urogenital sinus cells via the recombinant retrovirus Zipras/myc 9. After 4 weeks' growth as subcapsular renal grafts, poorly differentiated carcinomas are produced in C57BL/6 mice. In this study we examined the temporal relationships between morphological alterations, growth, DNA ploidy status and clonal selection as determined by Southern blotting in ras + myc-initiated carcinomas. Nuclear image analysis demonstrated that the emergence of a cycling DNA tetraploid cell population strongly correlated with growth and histologic progression. These tightly linked events culminated in the outgrowth of mono- or oligoclonal cancer. |
