| First Author | Dyck JR | Year | 1995 |
| Journal | J Biol Chem | Volume | 270 |
| Issue | 3 | Pages | 1375-81 |
| PubMed ID | 7836405 | Mgi Jnum | J:22398 |
| Mgi Id | MGI:70271 | Doi | 10.1074/jbc.270.3.1375 |
| Citation | Dyck JR, et al. (1995) Activation of the Na+/H+ exchanger gene by the transcription factor AP-2. J Biol Chem 270(3):1375-81 |
| abstractText | We have isolated and characterized regions important for expression of the mouse Na+/H+ exchanger gene. A 1.1-kilobase fragment upstream of the 5'-untranslated region contains specific DNA motifs characteristic of promoter and enhancer elements including a TATA box, two CAAT boxes, an SP-1 site, a cyclic AMP response element-binding site, and an AP-2-like site. This 1.1-kilobase fragment directs transcription of a luciferase reporter gene in mouse fibroblasts (NIH 3T3) and human Hep G2 cells. Deletion or mutation of an AP-2-like site 100 base pairs from the start site of transcription resulted in loss of most of the reporter plasmid activity. In addition, cotransfection of an AP-2 expression plasmid and the mouse promoter/luciferase plasmid increased the amount of Na+/H+ exchanger-directed transcription in AP-2-deficient Hep G2 cells. Moreover, mobility shift analysis indicated that a putative AP-2-binding site is capable of binding purified AP-2 protein and a specific protein from nuclear extracts of NIH 3T3 cells. The results show that the transcription factor AP-2 may play an important role in regulation of transcription of the mouse Na+/H+ exchanger gene. |
