| First Author | Zhdanova NS | Year | 1994 |
| Journal | Mamm Genome | Volume | 5 |
| Issue | 12 | Pages | 781-4 |
| PubMed ID | 7894159 | Mgi Jnum | J:22178 |
| Mgi Id | MGI:70060 | Doi | 10.1007/BF00292013 |
| Citation | Zhdanova NS, et al. (1994) Characterization of pig-mink cell hybrids: assignment of the TK1 and UMPH2 genes to pig chromosome 12. Mamm Genome 5(12):781-4 |
| abstractText | By fusion of thymidine kinase-deficient mink cells with pig leukocytes, a new type of cell hybrid was produced. It was demonstrated that pig chromosomes segregate in pig-mink hybrids and that hybrid cells contain no cytologically visible rearrangements between the chromosomes of parental species, or chromosome fragmentation. With a set of subclones of two primary hybrid clones, the genes for thymidine kinase-1 (TK1) and uridine 5'-monophosphate hydrolase-2 (UMPH2) were assigned to pig Chromosome (Chr) 12. A cell line with a single pig Chr 8 on the background of mink chromosomes was established. This clone could serve as a source of DNA for building a chromosome-specific library of pig Chr 8. The data obtained suggest that pig-mink cell hybrids can be used for mapping of pig chromosomes. |
