| First Author | Kest B | Year | 1994 |
| Journal | J Neurosci Res | Volume | 39 |
| Issue | 6 | Pages | 674-9 |
| PubMed ID | 7897702 | Mgi Jnum | J:22115 |
| Mgi Id | MGI:70001 | Doi | 10.1002/jnr.490390608 |
| Citation | Kest B, et al. (1994) Supraspinal delta opioid receptor mRNA levels are not altered in [D-Ala2]deltorphin II tolerant mice. J Neurosci Res 39(6):674-9 |
| abstractText | Delta opioid receptor (DOR) mRNA levels were studied in mice rendered tolerant to [D-Ala2]deltorphin II by 4 days of repeated intracerebroventricular administration (10 micrograms, [tid]). ED50 determinations on day 5 revealed a 10-fold loss in [D-Ala2]deltorphin II potency with the tail-flick test. Utilization of a microdissection technique followed by quantitative solution hybridization of RNA extracts from mouse brain revealed mean levels of DOR mRNA ranging from 3.9 pg/micrograms RNA in the caudate-putamen to 0.4 pg/micrograms RNA in the cerebellum. DOR mRNA levels were not different when RNA extracts from tolerant and non-tolerant mice were compared. These data suggest that altered DOR mRNA levels are not one of the adaptive changes that occur with delta opioid ([D-Ala2]deltorphin II) tolerance. |
