| First Author | Puel A | Year | 1995 |
| Journal | J Immunol | Volume | 154 |
| Issue | 11 | Pages | 5799-805 |
| PubMed ID | 7751629 | Mgi Jnum | J:25535 |
| Mgi Id | MGI:73251 | Doi | 10.4049/jimmunol.154.11.5799 |
| Citation | Puel A, et al. (1995) Mapping of genes controlling quantitative antibody production in biozzi mice. J Immunol 154(11):5799-5805 |
| abstractText | An extensive search for in vivo immunomodulatory genes (Im genes) was made, using highly polymorphic DNA markers (microsatellites), in F-2 hybrids between the two lines of mice selected for high (H) or low (L) Ab production (Biozzi mice). H and L mice have extreme phenotypes resulting from the accumulation, during selective breeding, of genes endowed with, respectively, upward or downward additive effects on Ab production. A total genome screening with 90 microsatellite markers (polymorphic between H and L mice) was conducted in 60 F-2 hybrids sorted out for their extreme phenotypes from an immunized population of 240 individuals. A difference in parental marker frequency between these two groups (measured by a chi(2) test) reveals the presence of an Im gene close to the marker. Significant chi(2) scores were indeed found in two regions corresponding to the MHC and Igh loci, al ready identified as partly contributing to the H/L phenotypic difference. A notable finding was the demonstration that a gene(s), located on the proximal part of chromosome 6, was linked with Ab responsiveness. Other markers at distinct chromosomal regions also give increased chi(2) scores, the two regions most clearly pointed out being on chromosomes 4 and 8. |
