| First Author | Schollen E | Year | 1995 |
| Journal | Biochem Biophys Res Commun | Volume | 211 |
| Issue | 1 | Pages | 115-22 |
| PubMed ID | 7779075 | Mgi Jnum | J:25965 |
| Mgi Id | MGI:73669 | Doi | 10.1006/bbrc.1995.1785 |
| Citation | Schollen E, et al. (1995) A regulatory element in the 5'UTR directs cell-specific expression of the mouse alpha 4 gene. Biochem Biophys Res Commun 211(1):115-22 |
| abstractText | Transfection experiments showed that the mouse alpha 4 promoter contains a downstream element in its 5'UTR which is essential for efficient promoter activity. DNaseI footprinting and electrophoretic mobility shift assays (EMSA) demonstrated that the region from nt +113 to +148 can bind a cell type-specific factor (MIII-3) present in the alpha 4m expressing cell line L1210 but not in the non-expressing cell lines A9 and LMTK. Two consensus SP1 sites in this 5'UTR were recognised in L1210, A9 and LMTK, and with extracts from A9 and LMTK, an AP2-like protein was shown to bind a downstream AP2 site. Thus both ubiquitous and cell type-specific factors regulate the expression of alpha 4m. |
