| First Author | Shi MM | Year | 1995 |
| Journal | Biochem Biophys Res Commun | Volume | 211 |
| Issue | 1 | Pages | 289-95 |
| PubMed ID | 7779098 | Mgi Jnum | J:25962 |
| Mgi Id | MGI:73666 | Doi | 10.1006/bbrc.1995.1809 |
| Citation | Shi MM, et al. (1995) Molecular cloning and posttranscriptional regulation of macrophage inflammatory protein-1 alpha in alveolar macrophages. Biochem Biophys Res Commun 211(1):289-95 |
| abstractText | Macrophage inflammatory protein-1 alpha (MIP-1 alpha) belongs to the chemokine superfamily of chemoattractant pro-inflammatory cytokines. MIP-1 alpha is chemotactic for monocytes and neutrophils and thus, plays an important role in initiation and control of inflammation. We have isolated and sequenced a cDNA clone encoding rat MIP-1 alpha. This 0.75 kb cDNA includes a single open reading frame of 92 amino acids. Expression of MIP-1 alpha mRNA was characterized in NR8383, a rat alveolar macrophage cell line (RAM). In resting RAM cells, MIP-1 alpha mRNA decayed rapidly, with a half life of less than 2 hours. Lipopolysaccharide (LPS) treatment of RAM cells resulted in a dose-dependent increase in MIP-1 alpha steady state mRNA expression. The induction of MIP-1 alpha mRNA by LPS was partially the result of mRNA stabilization, as half life increased to over 6 hours. |
