| First Author | Ogilvie P | Year | 1995 |
| Journal | FASEB J | Volume | 9 |
| Issue | 9 | Pages | 799-806 |
| PubMed ID | 7541381 | Mgi Jnum | J:26838 |
| Mgi Id | MGI:74268 | Doi | 10.1096/fasebj.9.9.7541381 |
| Citation | Ogilvie P, et al. (1995) Induction and variants of neuronal nitric oxide synthase type I during synaptogenesis. FASEB J 9(9):799-806 |
| abstractText | In the adult central nervous system, nitric oxide (NO) is formed from L-arginine by the so-called constitutive or type I NO synthase (NOS-I155). However, expression of NOS-I155 immunoreactivity and activity was low or not detectable in developing mouse and rat brain. NOS-I155 was sharply induced coincident with the onset of synaptogenesis in specific brain regions. This was followed by a second phase in which total NOS-I155 expression decreased both in specific cell populations and in the total synaptosomal subcellular fraction.Furthermore, two putative variants of NOS-I were transiently observed: an NOS-I-immunoreactive protein with increased electrophoretic mobility (NOS-I144) and a transient hypersensitivity of NOS-I155 to the competitive substrate inhibitor N omega-nitro-L-arginine. It is concluded that NOS-I expression is not constitutive but locally induced. In the central nervous system, this regionally specific, biphasic pattern of postnatal NOS-I induction is consistent with a role for NO in synaptogenesis and synaptic plasticity. |
