| First Author | Chireux MA | Year | 1995 |
| Journal | J Neurosci Res | Volume | 40 |
| Issue | 4 | Pages | 427-38 |
| PubMed ID | 7616604 | Mgi Jnum | J:23602 |
| Mgi Id | MGI:71185 | Doi | 10.1002/jnr.490400402 |
| Citation | Chireux MA, et al. (1995) Human choline acetyltransferase gene: localization of alternative first exons. J Neurosci Res 40(4):427-38 |
| abstractText | Two overlapping cosmids containing the 5' end of human choline acetyltransferase (ChAT) gene have been cloned. Using heterologous probes, we localized two alternative first exons homologous to rodent ChAT exons R and M (Misawa et al.: J Biol Chem 267:20392-20399, 1992). The sequence of rodent exon N was not conserved in the human gene. Northern blot analysis of mRNA purified from the human neuroepithelioma cell lines LA-N2 and MC-I-XC revealed that both exons R and M were transcribed in mRNA species of 6.0 and 2.5 kb. Only the 6-kb species was detected with both R- and M-specific probes in the neuroepithelioma cell line CHP126. Reverse transcription-polymerase chain reaction (RT-PCR) analysis suggested that the major mRNA species in MC-I-XC and CHP126 cells contained the proximal part of exon M spliced to exon 1, which contains the alternative ACG initiation codon. RT-PCR also allowed the characterization of a mRNA species containing exon R spliced to exon 1, but no species containing both exon R and the distal part of exon M could be detected. RT-PCR was also used to evidence an alternative exon (tentatively numbered exon 8) in the coding sequence. |
