| First Author | Hiratsuka A | Year | 1995 |
| Journal | Biochem Biophys Res Commun | Volume | 212 |
| Issue | 3 | Pages | 743-50 |
| PubMed ID | 7626107 | Mgi Jnum | J:27411 |
| Mgi Id | MGI:74854 | Doi | 10.1006/bbrc.1995.2032 |
| Citation | Hiratsuka A, et al. (1995) A subfamily 2 homo-dimeric glutathione S-transferase mYrs-mYrs of class theta in mouse liver cytosol. Biochem Biophys Res Commun 212(3):743-50 |
| abstractText | A homo-dimeric subfamily 2 glutathione (GSH) S-transferase (GST) mYrs-mYrs of the class theta was isolated from mouse liver cytosol and purified to homogeneity. The first 28 N-terminal amino acid sequence of the GST was completely identical to that of rat subfamily 2 GST Yrs-Yrs of the class theta. GST mYrs-mYrs cross-reacted with anti-rat GST Yrs-anti-sera but not with anti-sera raised against rat GSTs Ya-Ya (alpha), Yb1-Yb1 (mu), and Yp-Yp (pi) and represented more than 95% of the mouse liver cytosolic GST activity to scavenge the reactive sulfate ester 5-sulfoxymethylchrysene of the potent carcinogen 5-hydroxymethylchrysene. The mouse class theta GST had little activity toward 1-chloro-2,4-dinitrobenzene and was unretainable on GSH and an S-hexyl-GSH affinity columns. GST mYrs-mYrs had a much higher GSH peroxidase activity toward fatty acid hydroperoxides than did the other classes of mouse GSTs. |
