| First Author | Zhu W | Year | 1995 |
| Journal | Brain Res Mol Brain Res | Volume | 30 |
| Issue | 2 | Pages | 312-26 |
| PubMed ID | 7637581 | Mgi Jnum | J:25647 |
| Mgi Id | MGI:73361 | Doi | 10.1016/0169-328x(95)00017-m |
| Citation | Zhu W, et al. (1995) Id gene expression during development and molecular cloning of the human Id-1 gene. Brain Res Mol Brain Res 30(2):312-26 |
| abstractText | Id genes encode helix-loop-helix proteins that inhibit transcription by forming inactive heterodimers with basic helix-loop-helix (bHLH) proteins. bHLH proteins normally form either homodimers or heterodimers with other bHLH proteins and bind to a DNA sequence element activating transcription. Id-containing heterodimers are inactive because Id proteins lack the basic amino acid region necessary to form a DNA-binding domain. We have examined the relative levels of Id-1 and Id-2 mRNA during normal development and in malignant tissues. In the course of these experiments we cloned and sequenced the human Id-1 cDNA. Two related cDNA molecules encoding human Id-1 mRNAs were identified. Id-1a is a cDNA of 958 nucleotides and can encode a protein of 135 amino acids. Id-1b cDNA is 1145 nucleotides, can encode a protein of 149 amino acids, and appears to be a splice variant of Id-1a. The amino acid sequence of human Id-1 is greater than 90% homologous to that of mouse Id-1. The patterns of Id-1 and Id-2 expression during mouse development vary widely, and we detected Id-1 expression in human fetal and adult tissues from lung, liver, and brain. High Id-1 mRNA expression was found in many human tumor cell lines, including those isolated from nervous system tumors. We mapped Id-2 to human chromosome 2p25. |
