| First Author | Song JJ | Year | 1995 |
| Journal | Endocrinology | Volume | 136 |
| Issue | 10 | Pages | 4293-7 |
| PubMed ID | 7664647 | Mgi Jnum | J:47324 |
| Mgi Id | MGI:1203307 | Doi | 10.1210/endo.136.10.7664647 |
| Citation | Song JJ, et al. (1995) Bone morphogenetic protein-9 binds to liver cells and stimulates proliferation. Endocrinology 136(10):4293-7 |
| abstractText | A new member of the transforming growth factor (TGF)-beta superfamily, BMP-9, has recently been identified and shown to be expressed in the developing mouse liver. This report demonstrates that human HepG2 liver tumor cells bind recombinant human BMP-9 (rhBMP-9) with high affinity. Cross-linking analysis indicates that HepG2 cells express two BMP-9 receptors of approximately 54 and 80 kilodaltons, similar in size to the Type I and Type II receptors reported by others for TGF-beta and BMP-4. However, cross-competition experiments demonstrate that the BMP-9 receptors on HepG2 cells do not bind other BMPs or TGF-beta s, indicating that these are novel receptors with binding specificity for BMP-9. In functional studies, rhBMP-9 stimulates HepG2 cell proliferation as indicated by [3H]thymidine incorporation and cell counting assays. A proliferative effect of rh-BMP-9 was also observed on primary rat hepatocytes. In contrast, TGF-beta had no effect on HepG2 cell proliferation and inhibited proliferation in primary hepatocytes. These results suggest that BMP-9, acting through a novel set of receptors, may play a regulatory role in hepatic growth and function. |
