| First Author | Jones EP | Year | 1995 |
| Journal | Genomics | Volume | 27 |
| Issue | 1 | Pages | 40-51 |
| PubMed ID | 7665183 | Mgi Jnum | J:25623 |
| Mgi Id | MGI:73337 | Doi | 10.1006/geno.1995.1006 |
| Citation | Jones EP, et al. (1995) MHC class I gene organization in > 1.5-Mb YAC contigs from the H2-M region. Genomics 27(1):40-51 |
| abstractText | Sixteen yeast artificial chromosome (YAC) clones have been mapped to the H2-M region at the distal end of the mouse major histocompatibility complex (MHC) on chromosome 17. Analysis of the YACs with single- and multicopy probes yielded a proximal contig spanning a minimum of 800 kb and a distal contig of 700 kb. A probe for the conserved fourth exon of MHC class I genes detected 19 restriction fragments, including 6 of the 8 previously characterized H2-M class I genes, in the proximal contig. This contig spans the gap from the M to the T region and includes the T1 gene. By contrast, only two class I genes, M2 and M3, were found in the distal contig. These two genes, which are both expressed, may mark the end of the MHC. The order among nine class I genes and seven other markers was determined in the cloned DNA from the centromere as T1, Tu32A, (M1-M7-M8), Tu32B, B30, M6, M4, M5, Mog, Tu42A parallel M2, Leh525, M3, Tu42B, where the orientation with respect to the centromere is unknown for M1-M7-M8. |
