| First Author | Imagawa M | Year | 1995 |
| Journal | J Biol Chem | Volume | 270 |
| Issue | 36 | Pages | 20898-900 |
| PubMed ID | 7673112 | Mgi Jnum | J:28693 |
| Mgi Id | MGI:76237 | Doi | 10.1074/jbc.270.36.20898 |
| Citation | Imagawa M, et al. (1995) CTG triplet repeat in mouse growth inhibitory factor/metallothionein III gene promoter represses the transcriptional activity of the heterologous promoters. J Biol Chem 270(36):20898-900 |
| abstractText | Growth inhibitory factor/metallothionein III (GIF/MT-III) is expressed specifically in brain, and neither mRNA nor protein is detected in other organs. This tissue-specific expression might be regulated by negative elements as well as positive elements, such as tissue-specific enhancers. To investigate the repression mechanisms of this gene in organs other than the brain, transfection experiments were performed by using various deletion mutants. Interestingly, a 25 x CTG repeat in the promoter region seemed to contribute to the repression activity. Moreover, the repression activity of this 25 x CTG repeat was also observed in various promoters and in a direction and position independent manner, indicating that this element could act as a silencer. However, no binding protein was detected by gel-shift and footprint analyses. These results strongly suggest that the CTG repeat functions as a negative element, and that this effect is caused by unknown mechanisms, rather than by interactions between specific cis-elements and specific trans-acting factors as reported previously. It is also possible that the CTG repeat functions as a general silencer in many genes. |
