| First Author | Takahashi C | Year | 1995 |
| Journal | Biochem Biophys Res Commun | Volume | 215 |
| Issue | 2 | Pages | 538-46 |
| PubMed ID | 7487989 | Mgi Jnum | J:29280 |
| Mgi Id | MGI:76811 | Doi | 10.1006/bbrc.1995.2498 |
| Citation | Takahashi C, et al. (1995) Involvement of PIM-1 in DNA fragmentation in mouse NS-1-derived cells. Biochem Biophys Res Commun 215(2):538-46 |
| abstractText | In several cell lines derived from mouse NS-1 myeloma cells, internucleosomal fragmentation of chromosomal DNA, a hallmark of apoptosis, was continuously observed. Approximately 15-20% of the cells died when isolated in a 96-well plate, and the surviving cells contained fragmented DNA ('ladder'). Among a variety of genes so far reported to be related to apoptosis, only Pim-1 was expressed at an elevated level in the NS-1 hybridomas as compared in a control myeloma cell line without 'ladder'. Transfection of a Pim-1 expression vector to a 'ladder'-non-producing myeloma line yielded similar internucleosomal DNA fragmentation. The results hence suggested that Pim-1 activates endonucleases responsible for DNA fragmentation during apoptosis and/or repress DNA repair systems to restore fragmented DNA. |
