| First Author | Murray EJ | Year | 1995 |
| Journal | Miner Electrolyte Metab | Volume | 21 |
| Issue | 1-3 | Pages | 184-8 |
| PubMed ID | 7565446 | Mgi Jnum | J:30193 |
| Mgi Id | MGI:77707 | Citation | Murray EJ, et al. (1995) PTH-mediated osteoblast retraction: possible participation of the calpain pathway. Miner Electrolyte Metab 21(1-3):184-8 |
| abstractText | Parathyroid hormone (PTH)-induced osteoblast retraction may play a pivotal role a role in bone resorption by providing osteoclasts direct access to mineralized bone surface. We have been working on the hypothesis that the calpains participate in this retractile response through a calcium-dependent process. We have first demonstrated the presence of calpain activities in MC3T3-E1 osteoblastic cells and that these activities can be stimulated by PTH. Second, we have demonstrated that the PTH-induced osteoblast retraction is dramatically attenuated by two different cysteine protease inhibitors. Finally, initial immunofluorescent cytochemical studies suggest that this PTH-induced osteoblastic retraction is mediated through a calpain-dependent, proteolytic modification of the cytoskeletal organization. |
