| First Author | Hsu C | Year | 1995 |
| Journal | Brain Res Mol Brain Res | Volume | 32 |
| Issue | 2 | Pages | 241-51 |
| PubMed ID | 7500835 | Mgi Jnum | J:28375 |
| Mgi Id | MGI:75993 | Doi | 10.1016/0169-328x(95)00082-4 |
| Citation | Hsu C, et al. (1995) The first intron of the mouse neurofilament light gene (NF-L) increases gene expression. Brain Res Mol Brain Res 32(2):241-51 |
| abstractText | Neurofilament expression is developmentally and post-transcriptionally controlled. Using transient transfection assays in mouse L cells, we demonstrate that the expression of the mouse neurofilament light subunit (NF-L) is influenced by intron sequences. NF-L expression was decreased twenty fold upon deletion of the three intron sequences. Elements contained principally within a 350 bp region of intron 1 were responsible for enhanced NF-L expression. Enhancement of expression did not occur when intron I was placed 3' to a heterologous chloramphenicol acetyl transferase (CAT) gene whose expression was driven by NF-L 5' sequences. The intron enhancement of NF-L expression was not promoter-specific and also occurred with the mouse sarcoma virus (MSV) LTR promoter. These data suggest intron sequences may be important in regulating NF gene expression. |
