| First Author | Rettenberger G | Year | 1995 |
| Journal | Chromosome Res | Volume | 3 |
| Issue | 8 | Pages | 473-8 |
| PubMed ID | 8581299 | Mgi Jnum | J:29766 |
| Mgi Id | MGI:77285 | Doi | 10.1007/BF00713961 |
| Citation | Rettenberger G, et al. (1995) Mapping of murine YACs containing the genes Cea2 and Cea4 after B1-PCR amplification and FISH-analysis. Chromosome Res 3(8):473-8 |
| abstractText | PCR with primers specific for the murine B1 consensus sequence allows amplification of DNA from murine sources. We have used B1-PCR for amplifying yeast artificial chromosome (YAC) DNA which can be used to localize single YACs by fluorescence in situ hybridization. The genes for the pregnancy-specific glycoproteins Cea2 and Cea4, both belonging to the large carcinoembryonic antigen gene family, were localized by chromosomal in situ suppression hybridization of three YAC clones to murine chromosome 7A2-A3. This was facilitated by the use of the mouse lymphoma cell line WMP/WMP which contains nine pairs of Robertsonian fusion chromosomes. |
