| First Author | Kato H | Year | 1995 |
| Journal | Vet Immunol Immunopathol | Volume | 48 |
| Issue | 3-4 | Pages | 221-31 |
| PubMed ID | 8578682 | Mgi Jnum | J:32675 |
| Mgi Id | MGI:80164 | Doi | 10.1016/0165-2427(95)05441-8 |
| Citation | Kato H, et al. (1995) Molecular cloning of equine interleukin-1 alpha and -beta cDNAs. Vet Immunol Immunopathol 48(3-4):221-31 |
| abstractText | Equine interleukin-1 alpha (IL-1 alpha) and IL-1 beta were molecularly cloned to establish a basis for research on inflammatory and immune responses in the horse. Equine peripheral blood mononuclear cells (PBMC) were stimulated with lipopolysaccharide (LPS), and cDNA clones of equine IL-1 alpha and IL-1 beta covering the whole coding sequences were isolated from them. These equine IL-1 alpha and IL-1 beta clones contained open reading frames encoding 271 and 269 amino acids, respectively. The deduced amino acid sequence of equine IL-1 alpha showed 71.6% and 60.2% similarity with that of human and murine IL-1 alpha, respectively. Similarly, the amino acid sequence of equine IL-1 beta showed 66.7% and 61.8% similarity with that of human and murine IL-1 beta, respectively. In both equine IL-1 alpha and IL-1 beta, amino acids at miristoylation sites were well conserved. Dot blot analysis indicated that the expression of IL-1 beta was predominant to that of IL-1 alpha in equine PBMC stimulated with LPS or phorbol myristate acetate. |
