| First Author | Hunte BE | Year | 1996 |
| Journal | J Immunol | Volume | 156 |
| Issue | 2 | Pages | 489-96 |
| PubMed ID | 8543798 | Mgi Jnum | J:30540 |
| Mgi Id | MGI:78047 | Doi | 10.4049/jimmunol.156.2.489 |
| Citation | Hunte BE, et al. (1996) flk2/flt3 ligand is a potent cofactor for the growth of primitive B cell progenitors. J Immunol 156(2):489-96 |
| abstractText | We have investigated the role of flk2/flt3 ligand (FL) in B cell lymphopoiesis. The ability of FL to stimulate the growth of immature B cells was assessed using distinct populations: CD43lowB220+ pre-B cells, CD43+B220+ pro-B cells, and CD43+B220low progenitors. FL failed to affect the growth of the pro-B or pre-B cells whether used alone or in combination with stem cell factor (SCF) or IL-7. In striking contrast, FL was a potent cofactor for the CD43+B220low progenitor cells, interacting with either IL-7 and/or SCF to stimulate their growth. The combination of FL with IL-7 plus SCF stimulated maximum expansion of these cells, albeit, less than that stimulated in stromal cell cultures. When the CD43+B220low progenitors were divided based on expression of heat stable Ag (CD24) into a CD24- and a CD24+ subset, the FL-responsive cells were contained only within the CD24- subset. FL interacted with SCF or with IL-7 to stimulate their growth resulting in a 20- and 50-fold increase in cellularity, respectively. Since the CD24- subset was the most immature of the B cell populations studied, our data suggest that FL costimulates the expansion of very primitive B cell progenitors. |
