| First Author | Pronost S | Year | 1995 |
| Journal | Osteoarthritis Cartilage | Volume | 3 |
| Issue | 3 | Pages | 147-55 |
| PubMed ID | 8581744 | Mgi Jnum | J:32670 |
| Mgi Id | MGI:80159 | Doi | 10.1016/s1063-4584(05)80049-4 |
| Citation | Pronost S, et al. (1995) Modulation of interleukin-1 receptor expression by transforming growth factor-beta in cultured rabbit articular chondrocytes: analysis by reverse transcription-polymerase chain reaction. Osteoarthritis Cartilage 3(3):147-55 |
| abstractText | Interleukin-1 receptor type I (IL-1RI) expression in cultured rabbit articular chondrocytes (RAC) was studied by reverse transcription-polymerase chain reaction (RT-PCR). A cDNA probe specific for the rabbit IL-1RI gene was constructed using primers derived from the sequence data of the human, murine and chick receptors. Transforming growth factor-beta 1 (TGF beta-1) was shown to transiently increase the level of expected 900-bp PCR product at 1 h of incubation and decrease the expression at 48 and 72 h with no effect at 24 h. In receptor binding assays using [125I]-IL-1 alpha, TGF beta decreased IL-1R bioactivity at all time points. These results suggest that TGF beta-induced down-regulation of IL-1 RI could be responsible for its ability to antagonize the effect of IL-1 and that TGF beta may have a role in the repair of articular cartilage. |
