| First Author | Jin JP | Year | 1996 |
| Journal | Gene | Volume | 168 |
| Issue | 2 | Pages | 217-21 |
| PubMed ID | 8654947 | Mgi Jnum | J:31783 |
| Mgi Id | MGI:79269 | Doi | 10.1016/0378-1119(95)00803-9 |
| Citation | Jin JP, et al. (1996) Expression of cDNAs encoding mouse cardiac troponin T isoforms: characterization of a large sample of independent clones. Gene 168(2):217-21 |
| abstractText | We have isolated 52 mouse cardiac troponin-Tr-encoding cDNA clones (TnT) by specific antibody screening of a lambda ZAPII expression library. Sequencing data from the large sample of independent cDNAs demonstrated relationships among the expression of four alternatively- spliced exons of the cardiac TnT gene, producing seven classes of cDNAs encoding four protein isoforms differing in two variable regions. In the N-terminal variable region and next to the embryonic-specific exon 4, an alternatively spliced exon 3a was identified in 20% of the adult isoforms. The alternatively spliced exon 12, corresponding to a central variable, region between the two functional domains of TnT, was found in approx. 79% of the 52 mouse cardiac TnT cDNAs with a single base mutation completely abolishing the splicing at an internal acceptor site. Three novel alternative splicing acceptor sites in the 5'-untranslated portion of exon 2 have been identified with different frequencies. |
