| First Author | Wein D | Year | 1996 |
| Journal | Cancer Lett | Volume | 101 |
| Issue | 2 | Pages | 241-6 |
| PubMed ID | 8620476 | Mgi Jnum | J:32874 |
| Mgi Id | MGI:80360 | Doi | 10.1016/0304-3835(96)04146-8 |
| Citation | Wein D, et al. (1996) Expression of P-glycoprotein, encoded by MDR 1 gene, a metabolically active efflux pump in murine mast cells. Cancer Lett 101(2):241-6 |
| abstractText | Mast cell line (MC/9) derived from normal murine liver was examined for the expression of P-glycoprotein (P-gp) at the level of protein with C-219 and JSB-1 monoclonal antibodies, using flow cytometry and Western blot, and at the level of mRNA by the reverse transcriptase-polymerase chain reaction. The function of P-gp was analyzed by the accumulation and efflux of rhodamine 123 (Rh123) in the presence or absence of cyclosporin A (CSA) and a non-immunosuppressive analog of CSA (CSA-1). P-gp both at the protein and mRNA levels was expressed in mast cells. Intracellular accumulation of Rh123, in the presence of CSA and CSA-1 was significantly greater than in their absence. Furthermore, both CSA and CSA-1 inhibited Rh123 efflux from mast cells. These data suggest the presence of a functionally active P-gp in mast cells. A possible physiologic role for P-gp in the secretion of certain mediators/cytokines from mast cells is suggested. |
