| First Author | Botz J | Year | 1996 |
| Journal | Mol Cell Biol | Volume | 16 |
| Issue | 7 | Pages | 3401-9 |
| PubMed ID | 8668155 | Mgi Jnum | J:34288 |
| Mgi Id | MGI:81749 | Doi | 10.1128/mcb.16.7.3401 |
| Citation | Botz J, et al. (1996) Cell cycle regulation of the murine cyclin E gene depends on an E2F binding site in the promoter. Mol Cell Biol 16(7):3401-9 |
| abstractText | Cyclin E controls progression through the G1 phase of the cell cycle in mammalian fibroblasts and potentially in many other cell types. Cyclin E is a rate-limiting activator of cdk2 kinase in late G1. The abundance of cyclin E is controlled by phase-specific fluctuations in the mRNA level; in mammalian fibroblasts, mRNA is not detected under conditions of serum starvation and is accumulated upon serum stimulation, with expression starting in mid-G1. Here, we report the cloning of the murine cyclin E promoter. We isolated a 3.8-kb genomic fragment that contains several transcriptional start sites and confers cell cycle regulation on a luciferase reporter gene. This fragment also supports transcriptional activation by adenovirus E1A, a known upstream regulator of cyclin E gene expression. An E2F binding site which is required for G1-specific activation of the cyclin E promoter in synchronized NIH 3T3 cells was identified in this fragment. |
