| First Author | Yoshikawa T | Year | 1996 |
| Journal | Genomics | Volume | 35 |
| Issue | 2 | Pages | 361-6 |
| PubMed ID | 8661150 | Mgi Jnum | J:34727 |
| Mgi Id | MGI:82181 | Doi | 10.1006/geno.1996.0368 |
| Citation | Yoshikawa T, et al. (1996) New variants of the human and rat nuclear hormone receptor, TR4: expression and chromosomal localization of the human gene. Genomics 35(2):361-6 |
| abstractText | TRA is a new member of the nuclear hormone receptor family. This receptor is highly conserved in rat and human, but an in-frame insertion of 19 amino acid residues in the amino-terminal (A/B) region was found in the human homolog, which we refer to as hTR4 alpha 1. By reverse transcription-PCR (RT-PCR) we have identified a human TR4 mRNA (hTR4 alpha 2) that is analogous in size and sequence to the reported rat TRA. RT-PCR analysis using total RNA derived from various rat tissues revealed a new rat TR4 transcript, referred to as rTR4 alpha 1, which is homologous to hTR4 alpha 1 since it contains the extra 19 amino acids in the A/B region, The two rat transcripts showed a differential tissue distribution. Analysis of the exon-intron organization of the hTR4 A/B region showed that the 19-amino acid peptide insert in hTR4 alpha 1 was encoded by a separate exon, indicating that hTR4 alpha 1 and hTR4 alpha 2 transcripts were produced by the differential usage of the exon. RT-PCR analysis revealed that both hTR alpha 1 and hTR4 alpha 2 were detectable in brain, placenta, and ovary. In contrast, the human ovarian cancer cell Line, PA1, failed to express hTR4 alpha 1. By fluorescence in situ hybridization, we have mapped the hTR4 gene to 3p25, a region deleted in some forms of cancer. (C) 1996 Academic Press, Inc. |
