| First Author | Pleau JM | Year | 1996 |
| Journal | Biochem Biophys Res Commun | Volume | 224 |
| Issue | 3 | Pages | 747-53 |
| PubMed ID | 8713117 | Mgi Jnum | J:34496 |
| Mgi Id | MGI:81952 | Doi | 10.1006/bbrc.1996.1094 |
| Citation | Pleau JM, et al. (1996) Glutamic acid decarboxylase (GAD 67) gene expression in the pancreas and brain of the nonobese diabetic mouse. Biochem Biophys Res Commun 224(3):747-53 |
| abstractText | A quantitative PCR was developed to measure GAD 67 mRNA levels in pancreas from NOD mice. Two nonautoimmune mouse strains were used as controls. An internal standard, rat GAD 67 mRNA, was prepared to quantify the reaction. In NOD mice GAD 67 expression in the pancreas was in the order of 2 x 10(6) molecules/microgram of total RNA; this expression was five times less in the two control mouse strains, whatever the age. The overexpression of GAD 67 characterized in the NOD mouse could be restricted to the pancreas since similar analysis performed in mouse brain did not reveal a large difference between the three mouse strains studied. |
