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Publication : Immunodetection of serotonin transporter from mouse brain.

First Author  Ovalle S Year  1995
Journal  Neuroreport Volume  6
Issue  17 Pages  2353-6
PubMed ID  8747152 Mgi Jnum  J:31246
Mgi Id  MGI:78743 Doi  10.1097/00001756-199511270-00019
Citation  Ovalle S, et al. (1995) Immunodetection of serotonin transporter from mouse brain. Neuroreport 6(17):2353-6
abstractText  A DNA fragment encoding amino acid sequences from the amino terminal region of the mouse serotonin transporter was isolated and sequenced. This transporter is widely distributed throughout the mouse brain, as deduced by heminested reverse transcription-polymerase chain reaction (RT-PCR) assays. To identify the serotonin transporter protein, we have developed specific antibodies against a fusion protein containing its amino terminal region, a domain which shows a low degree of homology between the different neurotransmitter transporters. Western blot analysis of mouse brain membranes detected the serotonin transporter as a 71 kDa polypeptide.
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