| First Author | Pennica D | Year | 1996 |
| Journal | Cytokine | Volume | 8 |
| Issue | 3 | Pages | 183-9 |
| PubMed ID | 8833032 | Mgi Jnum | J:32758 |
| Mgi Id | MGI:80248 | Doi | 10.1006/cyto.1996.0026 |
| Citation | Pennica D, et al. (1996) Human cardiotrophin-1: protein and gene structure, biological and binding activities, and chromosomal localization. Cytokine 8(3):183-9 |
| abstractText | Cardiotrophin-1 (CT-1) is a new member of the interleukin- 6 cytokine family that was identified from a mouse embryoid body cDNA library by expression cloning. Mouse CT- 1 induces features of hypertrophy in neonatal rat cardiac myocytes and binds to and activates the leukaemia inhibitory factor/gp130 receptor complex, In this work we report the isolation and characterization of cDNA and genomic clones encoding human CT-1, These clones encode a 201 amino acid protein that is 80% identical to the mouse protein, Human CT-1 produced by transfection of the cDNA clones into mammalian cells induces the hypertrophy of neonatal rat cardiac myocytes. Human and mouse CT-1 bind to the leukaemia inhibitory factor receptor on both human and mouse cell lines indicating a lack of species specificity. No binding to the human oncostatin M specific receptor was detected. A 1.7 kb CT-1 mRNA is expressed in adult human heart, skeletal muscle, ovary, colon, prostate and testis and in fetal kidney and lung. The coding region of CT-1 is contained on three exons and is located on human chromosome 16p11.1-16p11.2. (C) 1996 Academic Press Limited |
