| First Author | Singer MS | Year | 1996 |
| Journal | J Immunol Methods | Volume | 196 |
| Issue | 2 | Pages | 153-61 |
| PubMed ID | 8841453 | Mgi Jnum | J:35568 |
| Mgi Id | MGI:83014 | Doi | 10.1016/0022-1759(96)00106-8 |
| Citation | Singer MS, et al. (1996) Purification and quantification of L-selectin-reactive GlyCAM-1 from mouse serum. J Immunol Methods 196(2):153-61 |
| abstractText | GlyCAM-1 is an endothelial-derived mucin found in the conditioned medium of organ-cultured lymph nodes and in serum. It is a ligand for L-selectin, but also interacts with P and E-selectin. It may play a role in the modulation of leukocyte trafficking into secondary lymphoid tissues as well as chronic inflammatory sites. Here we report the development of an ELISA that quantifies GlyCAM-1 in complex biological fluids such as serum. The ELISA response is linear over a concentration range of 13-800 ng/ml. The level of GlyCAM-1 in mouse serum was found to be 1.3-1.6 micrograms/ml. Using mouse serum as starting material, we report a rapid and efficient purification scheme for the isolation of biologically active GlyCAM-1. This material may now be used to address the biological functions of GlyCAM-1 and to further define its structural features. |
