| First Author | Chastant S | Year | 1996 |
| Journal | Mol Reprod Dev | Volume | 44 |
| Issue | 4 | Pages | 423-32 |
| PubMed ID | 8844684 | Mgi Jnum | J:34632 |
| Mgi Id | MGI:82087 | Doi | 10.1002/(SICI)1098-2795(199608)44:4<423::AID-MRD1>3.0.CO;2-N |
| Citation | Chastant S, et al. (1996) Quantitative control of gene expression by nucleocytoplasmic interactions in early mouse embryos: consequence for reprogrammation by nuclear transfer. Mol Reprod Dev 44(4):423-32 |
| abstractText | HSP 70.1 is one of the first genes to be expressed in the mouse embryo at the time of zygotic genome activation. We studied the regulation of this gene, using a transgene associating HSP 70.1 promoter and the firefly luciferase reporter gene, which allows the precise quantification of HSP 70.1 level of expression on individual embryos. In the present work, we show first that the level of HSP 70.1 expression at the two-cell stage is significantly higher (around two-fold) in embryos whose maternal cytoplasm is from C3H strain than with BALB/c strain. We verified that this difference is not an artefact of the use of transgenic embryos, of the time of first cleavage, or of in vitro culture. This regulation of HSP 70.1 level of expression is controlled by strain-specific maternal modifiers and is independent of replication, syngamy, and mitosis. Following nuclear transfer, reactivation of HSP 70.1 is also subjected to the same epigenetic influence. Only the strain-of-origin of the recipient cytoplast modulates the level of HSP 70.1 reprogrammation; the origin of donor nucleus is not significant, demonstrating the reversibility of this strain effect. These results point out the importance of the quality of recipient cytoplast in the intensity of gene reprogrammation, which may be of importance for nuclear transfer efficiency. |
