| First Author | Bradley MP | Year | 1996 |
| Journal | Mol Reprod Dev | Volume | 44 |
| Issue | 4 | Pages | 452-9 |
| PubMed ID | 8844687 | Mgi Jnum | J:34631 |
| Mgi Id | MGI:82086 | Doi | 10.1002/(SICI)1098-2795(199608)44:4<452::AID-MRD4>3.0.CO;2-K |
| Citation | Bradley MP, et al. (1996) Cloning, sequencing, and characterization of LDH-C4 from a fox testis cDNA library. Mol Reprod Dev 44(4):452-9 |
| abstractText | A full-length cDNA encoding the sperm-specific enzyme lactate dehydrogenase-C4 was isolated from a fox testis cDNA expression library and sequenced. The deduced translated protein sequence was shown to be 86% identical to that of human LDH-C4. In the fox testis, mRNA encoding LDH-C4 was first detected in pachytene spermatocytes. The LDH-C4 protein monomer was identified in Western blots of sperm membrane extracts as having a molecular weight of approximately 35,000, consistent with the monomeric size of this subunit previously identified in sperm from other species. The LDH-C4 protein is localized on the sperm plasma membrane overlying the principal piece of the tail. Based on the available sequence data, we were able to identify an epitope within the N-terminal region of the LDH-C4 amino-acid sequence which when administered to female foxes is antigenic and produces antibodies capable of recognizing the native protein. |
