| First Author | Zhao QZ | Year | 1996 |
| Journal | Mamm Genome | Volume | 7 |
| Issue | 10 | Pages | 749-53 |
| PubMed ID | 8854862 | Mgi Jnum | J:35908 |
| Mgi Id | MGI:83351 | Doi | 10.1007/s003359900225 |
| Citation | Zhao QZ, et al. (1996) Cloning and characterization of the mouse alpha globin cluster and a new hypervariable marker. Mamm Genome 7(10):749-53 |
| abstractText | A 95-kb region of the mouse genome spanning the entire alpha-globin gene cluster was isolated as overlapping cosmid clones and characterized. In addition to the embryonic (zeta) and adult (ct) genes, the cloned contig contains the complete N-methylpurine-DNA glycosylase (MPG) gene, the alpha-globin-positive regulatory element (mHS- 26), and a previously unidentified hypervariable region (named the mouse alpha-KVR). In mice, the distance between the MPG gene and mHS-26 is similar to 18 kb; between the mHS-26 and the zeta-gene, similar to 26 kb; from the zeta- gene to the 5' end of the alpha-gene, similar to 16 kb; and the two cc-genes are separated by similar to 12 kb. In human, the corresponding distances are similar to 27 kb, similar to 40 kb, similar to 19 kb, and similar to 3 kb respectively. The alpha-HVR is located similar to 18 kb upstream of the mouse zeta-globin gene transcription start site and contains a variable copy number tandem repeat (VNTR) array of a 35-bp sequence rich in (G+C) content, The unit sequence of the HVR shares the shea core sequence with the HVRs identified in the human alpha-gene cluster. Thus, this HVR may be a valuable evolutionary marker, as well as a useful genetic marker for the mouse. |
