| First Author | Otte K | Year | 1996 |
| Journal | Gen Comp Endocrinol | Volume | 102 |
| Issue | 1 | Pages | 11-5 |
| PubMed ID | 8860303 | Mgi Jnum | J:32942 |
| Mgi Id | MGI:80430 | Doi | 10.1006/gcen.1996.0040 |
| Citation | Otte K, et al. (1996) Cloning and sequencing of an equine insulin-like growth factor I cDNA and its expression in fetal and adult tissues. Gen Comp Endocrinol 102(1):11-5 |
| abstractText | A cDNA for equine insulin-like growth factor I (IGF I) has been isolated by reverse transcriptase-polymerase chain reaction and subsequently sequenced. The sequenced fragment contained 465 bp including the coding regions for the signal peptide, the entire mature protein, and 4 amino acids into the E-peptide. Like its human counterpart, the mature equine IGF I peptide contains 70 amino acids and was 100% homologous between horse and man. The 49-amino-acid signal peptide had the threonine in position 26 of the human signal peptide substituted by isoleucine. The nucleotide homology across the entire clone was 96.3% between horse and man and 91.6% between horse and rat. The isolated cDNA hybridized to the same transcripts in fetal and adult tissues. |
