| First Author | McKay BS | Year | 1996 |
| Journal | J Biol Chem | Volume | 271 |
| Issue | 48 | Pages | 30544-7 |
| PubMed ID | 8940024 | Mgi Jnum | J:36811 |
| Mgi Id | MGI:84224 | Doi | 10.1074/jbc.271.48.30544 |
| Citation | McKay BS, et al. (1996) Molecular requirements for assembly and function of a minimized human integrin alphaIIbbeta3. J Biol Chem 271(48):30544-7 |
| abstractText | Integrin subunit compatibility within and between species plays a major role in heterodimer assembly and ligand specificity. As an example, human alphaIIb pairs only with human beta3 and does not assemble a heterodimer with beta3 from other species. We use interspecies subunit chimeras to identify molecular requirements for subunit compatibility and show that species-restricted heterodimer assembly depends on a unique hexapeptide VGSDNH in an extended loop of the hypothetical human beta3 MIDAS domain. This allows us to express alphaIIb(1-233) and beta3(111-318) as a soluble, mini-integrin that retains RGD-dependent ligand recognition. Thus, in the case of one integrin, alphaIIbbeta3, the molecular requirements for integrin subunit compatibility and ligand recognition are intimately related. |
