| First Author | Takagaki Y | Year | 1996 |
| Journal | Cell | Volume | 87 |
| Issue | 5 | Pages | 941-52 |
| PubMed ID | 8945520 | Mgi Jnum | J:36814 |
| Mgi Id | MGI:84227 | Doi | 10.1016/s0092-8674(00)82000-0 |
| Citation | Takagaki Y, et al. (1996) The polyadenylation factor CstF-64 regulates alternative processing of IgM heavy chain pre-mRNA during B cell differentiation. Cell 87(5):941-52 |
| abstractText | The switch from membrane-bound to secreted-form IgM that occurs during differentiation of B lymphocytes has long been known to involve regulated processing of the heavy chain pre-mRNA. Here, we show that accumulation of one subunit of an essential polyadenylation factor (CstF-64) is specifically repressed in mouse primary B cells and that overexpression of CstF-64 is sufficient to switch heavy chain expression from membrane-bound (microm) to secreted form (micros). We further show that CstF-64 is limiting for formation of intact CstF, that CstF has a higher affinity for the microm poly(A) site than for the micros site, and that the microm site is stronger in a reconstituted in vitro processing reaction. Our results indicate that CstF-64 plays a key role in regulating IgM heavy chain expression during B cell differentiation. |
