| First Author | Lin LH | Year | 1996 |
| Journal | Anat Rec | Volume | 246 |
| Issue | 4 | Pages | 535-44 |
| PubMed ID | 8955792 | Mgi Jnum | J:37947 |
| Mgi Id | MGI:85341 | Doi | 10.1002/(SICI)1097-0185(199612)246:4<535::AID-AR12>3.0.CO;2-P |
| Citation | Lin LH, et al. (1996) Sex-dependent expression of placental (P)-cadherin during mouse gonadogenesis. Anat Rec 246(4):535-44 |
| abstractText | BACKGROUND: Placental (P)-cadherin is one of a family of cell adhesion molecules that participate in embryonic sorting and organogenesis. In previous work, P-cadherin was localized to Sertoli cells in the mouse testis as early as postnatal day 1. This early postnatal localization raised questions about when P-cadherin first appeared in the embryonic testis and whether P-cadherin was expressed differentially in the embryonic testis and ovary. METHODS: The localization of P-cadherin, epithelial (E)-cadherin, and Mullerian inhibiting substance was determined in frozen sections of mouse gonads between embryonic days 10.5 and 18 using indirect immunohistochemistry. Alkaline phosphatase reactivity was used to identify germ cells. RESULTS: The expression of P-cadherin was traced back to the indifferent stage of gonadogenesis where uniform distribution was observed in the indifferent gonad of both sexes. However, after sexual differentiation, the expression of P-cadherin in the testis was localized to Sertoli cells in the testicular cords, while its expression in the ovary fell below detectable levels. CONCLUSIONS: The localization of P-cadherin in the male and female indifferent gonad is similar and cannot be used to distinguish the future testis and ovary. The localization of P-cadherin in the testis after sexual differentiation suggests a role for P-cadherin in testicular cord formation. The common temporal pattern of P-cadherin and Mullerian inhibiting substance expression in Sertoli cells is consistent with a shared regulatory mechanism. |
