| First Author | Howard AD | Year | 1996 |
| Journal | Cell Immunol | Volume | 174 |
| Issue | 2 | Pages | 155-64 |
| PubMed ID | 8954615 | Mgi Jnum | J:37123 |
| Mgi Id | MGI:84551 | Doi | 10.1006/cimm.1996.0305 |
| Citation | Howard AD, et al. (1996) Alteration of macrophage responsiveness to platelet-activating factor by interferon-gamma and lipopolysaccharide. Cell Immunol 174(2):155-64 |
| abstractText | Platelet-activating factor (PAF) can modulate several macrophage responses associated with tumoricidal and inflammatory activity. To determine how macrophage responsiveness to PAF may be altered by interferon-gamma (IFN-gamma) or lipopolysaccharide (LPS), we studied PAF receptor-associated activities. Pretreatment of murine peritoneal macrophages with either LPS or IFN-gamma suppressed macrophage responsiveness to both PAF-induced calcium mobilization and superoxide anion (O2-) production. This suppression of macrophage responsiveness to PAF was maximal when 25 U/ml IFN-gamma or 100 ng/ml LPS was initially added for 6 hr. Macrophages pretreated with LPS or IFN-gamma remained refractory to PAF-induced rise in intracellular calcium for 4 to 24 hr. Macrophages preincubated with 25 U/ml IFN-gamma remained refractory to PAF-induced calcium mobilization for up to 4 hr. LPS and IFN-gamma treatment also decreased PAF-induced, calcium-dependent O2- production. When added together, IFN-gamma increased the suppression of PAF-induced intracellular calcium mobilization and inhibited O2- production mediated by LPS. To assess whether suppression was mediated through altered PAF receptors, binding affinities were determined; two binding affinities were demonstrated. Initial incubation of macrophages with LPS or IFN-gamma added alone or together decreased the number of cell surface PAF receptors and their binding affinity. These studies demonstrated that pretreatment with IFN-gamma and LPS can suppress select PAF-induced macrophage functions. Downregulation of PAF receptor activity may represent a means by which macrophages regulate the capacity and magnitude of some PAF-induced responses. |
