| First Author | Squire JA | Year | 1996 |
| Journal | Cytogenet Cell Genet | Volume | 75 |
| Issue | 2-3 | Pages | 164-6 |
| PubMed ID | 9040785 | Mgi Jnum | J:39035 |
| Mgi Id | MGI:86419 | Doi | 10.1159/000134470 |
| Citation | Squire JA, et al. (1996) Allelic fusion of DNA topoisomerase II alpha and retinoic acid receptor alpha genes in adriamycin-resistant p388 murine leukemia revealed by fluorescence in situ hybridization. Cytogenet Cell Genet 75(2-3):164-6 |
| abstractText | Fluorescence in situ hybridization (FISH) analysis of metaphase and decondensed free chromatin fibers from Adriamycin (ADR)-sensitive and ADR-resistant murine cells demonstrated a close juxtaposition of topoisomerase II alpha (Top2a) and retinoic acid receptor alpha (Rara) genes in adjacent chromatin in the drug-resistant cells, and a close but separate genetic proximity in normal murine chromatin. This provides physical evidence that the chromosome 11 allelic rearrangement resulting in a chimeric truncated Top2a/Rara transcript in the ADR- resistant cells is due to a novel fusion of the Topo2a and Rara genes. This is the first description of a Rara gene disruption in cells selected for antineoplastic drug resistance. |
