| First Author | Sax CM | Year | 1997 |
| Journal | Gene | Volume | 185 |
| Issue | 2 | Pages | 209-16 |
| PubMed ID | 9055817 | Mgi Jnum | J:39066 |
| Mgi Id | MGI:86447 | Doi | 10.1016/s0378-1119(96)00643-9 |
| Citation | Sax CM, et al. (1997) Transcriptional regulation of the mouse alpha A-crystallin gene: binding of USF to the -7/+5 region. Gene 185(2):209-16 |
| abstractText | Lens preferred-expression of the mouse alpha A-crystallin gene (alpha A-cry) is regulated at the transcriptional level by multiple elements located in the 5' flanking region of the gene. Here we present the first analysis of the functional role of the mouse alpha A-cry +1 region and the protein(s) which bind to it. The -7/+5 region of this promoter exhibits sequence similarity with the consensus upstream stimulating factor (USF) transcription factor binding site. A wild type oligodeoxyribonucleotide (oligo) spanning the mouse alpha A-cry -15/+15 region specifically inhibited the activity of a mouse alpha A-cry promoter-cat gene fusion (p alpha A111(a)CAT) in competitive co- transfection studies in the mouse alpha TN4-1 lens cell line, as did an oligo containing the adenovirus 2 major late promoter strong USF binding site. In contrast, an alpha A-cry oligo mutated (-3/+3) within the USE-like binding site did not inhibit p alpha A111(a)CAT activity. Western blot analysis indicated that alpha TN4-1 cells express USF1. Co-transfection of p alpha A111(a)CAT and a USF1 cDNA expression vector into alpha TN4-1 cells resulted in a repression of mouse alpha A-cry promoter activity. Electrophoretic mobility shift analyses (EMSA) demonstrated that proteins in an alpha TN4-1 nuclear extract form a single major complex on synthetic oligos |
