| First Author | Edmiston JS | Year | 1997 |
| Journal | Int Immunol | Volume | 9 |
| Issue | 3 | Pages | 427-33 |
| PubMed ID | 9088981 | Mgi Jnum | J:39220 |
| Mgi Id | MGI:86602 | Doi | 10.1093/intimm/9.3.427 |
| Citation | Edmiston JS, et al. (1997) A transforming growth factor-beta regulable RNA-binding protein interacts specifically with germline Ig alpha transcripts. Int Immunol 9(3):427-33 |
| abstractText | Isotype switching is presaged by the transcriptional activation of the heavy chain class gene (CH) to which recombination will occur. As a result, mRNA or germline transcripts from the unrearranged gene accumulate in the cytoplasm. Previous studies demonstrated that transforming growth factor (TGF)-beta stimulated isotype switching to IgA in cultures of lipopolysaccharide (LPS)-stimulated murine B cells and increased the stability of C alpha mRNAs. The present study demonstrates that LPS-stimulated B cells express a 45 kDa protein, I alpha BP, that specifically binds to germline alpha transcripts. Following addition of TGF-beta, the binding activity of this protein is significantly reduced. The identification of a cytokine regulable RNA-binding protein that interacts with germline transcripts supports the idea that these transcripts are involved in recombination and raises the possibility that RNA-protein interactions play a role in regulating isotype switching. |
