| First Author | Mirahmadi R | Year | 1997 |
| Journal | C R Acad Sci III | Volume | 320 |
| Issue | 1 | Pages | 13-8 |
| PubMed ID | 9099259 | Mgi Jnum | J:41568 |
| Mgi Id | MGI:894055 | Doi | 10.1016/s0764-4469(99)80081-x |
| Citation | Mirahmadi R, et al. (1997) Effect of c-Fos overexpression on the murine macrophage cell line P388DI. C R Acad Sci III 320(1):13-8 |
| abstractText | In order to study the role of Fos on the regulation of proliferation in the monocyte-macrophage lineage we realized a stable transfection of the murine P388D1 cell line by the murine c-fos gene under the control of the human metallothionein IIA promoter. Several clones have been selected by geneticin: they show a variable number of integrated transgene (two to ten copies). Their expression has been analyzed in the presence or absence of cadmium chloride as inducer (5 x 10(-6) M). In one clone especially, the c-fos mRNA and Fos protein levels were respectively 6- and 10-fold increased. The study of cell growth by tritiated thymidine incorporation indicates a negative effect of the overexpressed Fos protein in the absence of any other stimulus. |
