| First Author | Komura Ji | Year | 1997 |
| Journal | J Biol Chem | Volume | 272 |
| Issue | 16 | Pages | 10975-80 |
| PubMed ID | 9099757 | Mgi Jnum | J:39583 |
| Mgi Id | MGI:86937 | Doi | 10.1074/jbc.272.16.10975 |
| Citation | Komura Ji, et al. (1997) In vivo ultraviolet and dimethyl sulfate footprinting of the 5' region of the expressed and silent Xist alleles. J Biol Chem 272(16):10975-80 |
| abstractText | The Xist (X inactive specific transcript) gene plays an essential role in X chromosome inactivation. To elucidate the mechanisms controlling Xist expression and X inactivation, we examined in vivo DNA-protein interactions in the Xist promoter region in a female mouse cell line (BMSL2), which has distinguishable Xist alleles. In vivo footprinting was accomplished by treatment of cells with dimethyl sulfate or ultraviolet light, followed by ligation-mediated polymerase chain reaction of purified DNA. The expressed allele on the inactive X chromosome and the silent allele on the active X chromosome were separated by the use of a restriction fragment length polymorphism prior to ligation-mediated polymerase chain reaction. The chromatin structure of the Xist promoter was found to be consistent with the activity state of the Xist gene. The silent allele (on the active X chromosome) showed no footprints, while the expressed allele (on the inactive X chromosome) showed footprints at a consensus sequence for a CCAAT box, two weak Sp1 sites, and a weak TATA box. |
