| First Author | Schiestl RH | Year | 1997 |
| Journal | Proc Natl Acad Sci U S A | Volume | 94 |
| Issue | 9 | Pages | 4576-81 |
| PubMed ID | 9114032 | Mgi Jnum | J:40163 |
| Mgi Id | MGI:87507 | Doi | 10.1073/pnas.94.9.4576 |
| Citation | Schiestl RH, et al. (1997) Carcinogens induce reversion of the mouse pink-eyed unstable mutation. Proc Natl Acad Sci U S A 94(9):4576-81 |
| abstractText | Deletions and other genome rearrangements are associated with carcinogenesis and inheritable diseases. The pink-eyed unstable (pun) mutation in the mouse is caused by duplication of a 70-kb internal fragment of the p gene. Spontaneous reversion events in homozygous pun/pun mice occur through deletion of a duplicated sequence. Reversion events in premelanocytes in the mouse embryo detected as black spots on the gray fur of the offspring were inducible by the carcinogen x-rays, ethyl methanesulfonate, methyl methanesulfonate, ethyl nitrosourea, benzo[a]pyrene, trichloroethylene, benzene, and sodium arsenate. The latter three carcinogens are not detectable with several in vitro or in vivo mutagenesis assays. We studied the molecular mechanism of the carcinogen-induced reversion events by cDNA analysis using reverse transcriptase-PCR method and identified the induced reversion events as deletions. DNA deletion assays may be sensitive indicators for carcinogen exposure. |
