| First Author | Chung WK | Year | 1997 |
| Journal | Obes Res | Volume | 5 |
| Issue | 3 | Pages | 183-5 |
| PubMed ID | 9192391 | Mgi Jnum | J:41295 |
| Mgi Id | MGI:893465 | Doi | 10.1002/j.1550-8528.1997.tb00291.x |
| Citation | Chung WK, et al. (1997) Polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) and electrophoretic assays for the mouse obese (Lepob) mutation. Obes Res 5(3):183-5 |
| abstractText | Three polymerase chain reaction-based assays for the mouse Lep(ob) mutation are described: Dde I: site created by the C-->T transversion characterizing Lep(ob) enables positive detection of the mutant allele; positive detection of the wild-type Lep allele is achieved by the use of primer sequence which introduces an A-->C substitution, creating an Msp I site in the normal allele; and an electrophoretic assay which positively identifies the heterozygote. |
